Leaf Volatiles and Relevant Gene Expression as the Specific Characteristics in Citrus depressa Accession Discrimination

Citrus depressa Hayata is the native and widespread citrus species in Taiwan. The notable character that C. has a distinct aroma different from local citrus. ex situ germplasm of scions collection regions variant leaf shapes odor characteristics. Establishing volatile biomarkers for classifying beneficial to commercial development. Volatile organic compounds (VOCs) fresh leaves seven accessions which were collected locations Taiwan extracted by headspace solid-phase microextraction analyzed GC-MS. compositions each season showed diversity, linalool, average relative content 52.7%, was most component any season. other main VOCs ?-terpinene, limonene, ?-ocimene, ?-terpineol. result linear discriminant analysis VOC markers shows there are two types (1) central east (2) closer Okinawa, Japan. Five major VOC-related synthase genes selected gene expression used classify varieties. clustering same with VOC-based discrimination. Our results reveal profiling capable being discrimination markers, possibility constructing molecular directly related characteristics secondary metabolites phenotyping.